Abcam, ab245514, Anti-p107 antibody

Size: 100µL
Rabbit Polyclonal p107 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RBL1 aa 1000 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RBL1 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P28749

Product details:
ab245514 has not performed satisfactorily when used for WB of p107 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of p107.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab245514 was affinity purified using an epitope specific to p107 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein p107 also known as Retinoblastoma-like protein 1 (RBL1) is a member of the retinoblastoma (RB) protein family and has a molecular weight of approximately 107 kDa. p107 is known for its role as a cell cycle regulator specifically in controlling cell cycle progression at the G1 phase. It is widely expressed in many tissues including the brain skeletal muscle and lymphoid tissues. Like other members of the RB family p107 contains an RB pocket domain important for its interactions with other proteins.
Biological function summary
Retinoblastoma-like protein 1 functions in the regulation of the cell cycle acting as an important checkpoint controller between the G1 and S phases. This protein forms a complex with E2F transcription factors which are important in controlling genes involved in cell cycle progression. The p107-E2F interaction represses transcription and inhibits the cell from proceeding to the S phase thereby ensuring proper cellular proliferation control. Moreover p107 can bind to cyclin-dependent kinases adding another layer of regulation.
Pathways
P107 plays essential roles in the RB-E2F pathway and the DNA damage response pathway. Within these pathways p107 interacts with other key proteins like p130 and pRB which together form the pocket protein family. The RB-E2F pathway controls cell cycle entry and exit while the DNA damage response pathway ensures genomic integrity. p107 acts as a mediator to pause the cell cycle during DNA damage repair collaborating with these related proteins to maintain cellular homeostasis.
P107 shows notable implications in cancer and retinoblastoma. Alterations in the expression or function of p107 can lead to unregulated cell proliferation contributing to oncogenesis. In cancer disruptions in the RB-E2F pathway often involving mutations or changes in p107 pRB or related proteins can induce tumor formation. In retinoblastoma although pRB plays a more dominant role reduced functionality of p107 can exacerbate the disease by weakening cell cycle control. Therefore p107 occupies an essential position in both safeguarding against diseases and being a potential factor in disease progression when dysregulated.