Abcam, ab245705, Human NT-ProANP ELISA Kit

Size: 1 x 96Tests
Human NT-ProANP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NT-ProANP with a sensitivity of 26.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 26.2 pg/mL,
Range:78.1 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human NT-ProANP ELISA Kit ab245705 is a rapid single-wash 90-min Sandwich ELISA to measure Human NT-ProANP in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 26.2 pg/mL.
How the assay works
Human NT-ProANP SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human NT-ProANP ELISA Kit ab245705 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Natriuretic peptides A antibody [EPR20247-105] - BSA and Azide free (Capture)
ab252629
- Anti-Natriuretic peptides A antibody [EPR22107-29] - BSA and Azide free (Detector)
ab252629

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Natriuretic Peptides A also known as ANP ANF or NPPA are important cardiac hormones made by atrial myocytes in the heart. This peptide with a mass of around 28 amino acids plays an important role mechanically in modulating blood pressure and fluid balance. It achieves this by binding to receptors that activate cyclic GMP (cGMP) production leading to vasodilation. ANP expression is mainly found in cardiac muscle cells but also detected in other tissues under certain conditions.
Biological function summary
These peptides contribute to renal sodium excretion commonly known as natriuresis and also promote diuresis which increases urine volume. ANP functions as a singular hormone without forming part of a complex. Beyond its role in cardiovascular and renal systems ANP influences lipid metabolism and adipocyte functions which may have implications for metabolic health.
Pathways
ANP is engaged in the natriuretic peptide signaling pathway which is important for cardiovascular homeostasis. Within this pathway related proteins include B-type Natriuretic Peptide (BNP) and C-type Natriuretic Peptide (CNP) all of which share similar receptor interactions through cGMP. Additionally ANP impacts the renin-angiotensin system (RAS) a significant hormonal system regulating blood pressure and fluid balance by counteracting the actions of angiotensin II.
ANP is notably associated with heart failure where its levels can indicate disease severity and prognosis. Elevated ANP levels have also been linked to hypertension contributing to diagnostic evaluations alongside other biomarkers like BNP. Both heart failure and hypertension involve complex interactions between ANP and proteins such as angiotensin II within their pathophysiological frameworks.