Abcam, ab245715, Human FLRG ELISA Kit

Size: 1 x 96Tests
Human FLRG ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FLRG with a sensitivity of 8.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Heparin Plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.2 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human FLRG ELISA Kit (ab245715) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FLRG protein in edta plasma, hep plasma, milk, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FLRG with 8.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein FSTL3 also known as Follistatin-like 3 has a molecular mass of approximately 26 kDa. Mechanically FSTL3 acts by binding and neutralizing specific members of the TGF-beta superfamily influencing cellular processes like growth differentiation and apoptosis. It is mostly expressed in tissues such as the placenta pancreas and muscle where it plays a role in various developmental and physiological events.
Biological function summary
FSTL3 serves an important function in regulating cellular dynamics by modulating activity of ligands in the TGF-beta signaling pathway. It forms a complex with activins and other growth factors to inhibit their signaling capabilities. This interaction is key in processes like managing fetal development ensuring proper organogenesis and maintaining metabolic activities.
Pathways
Extensive research places FSTL3 within the TGF-beta signaling pathway and the insulin-like growth factor (IGF) system. These pathways are critical for managing cellular growth and metabolism. FSTL3 interacts with proteins such as activins and inhibins which share roles in regulating cell proliferation and differentiation linking FSTL3 directly to these essential cellular pathways.
FSTL3 links to pathologies like type 2 diabetes and muscle wasting. FSTL3 influences insulin signaling therefore its dysregulation can contribute to insulin resistance a hallmark of type 2 diabetes. It also associates with muscle protein wasting through its interaction with activin suggesting a potential therapeutic target in conditions like cachexia.