Abcam, ab245880, H&E Staining Kit (Hematoxylin and Eosin)

Size: 30mL / 500mL
H&E Staining Kit (Hematoxylin and Eosin) ab245880 is intended for use in histology (fixed paraffin embedded or frozen tissue section) and cytology applications. - Uses improved Eosin formulation: lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section - Hematoxylin solution provides optimal contrast to Eosin stained cytoplasm - Cited in over 220 publications
Key facts
Sample types:Tissue sections

Product details:
How the assay works
Included in H&E Staining Kit (Hematoxylin and Eosin) is a newly formulated Eosin that provides the benefits of a traditional alcoholic formula with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, reduced tendency to spill over container, hands, and countertops, and improved surface tension to remain on tissue section. The Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.
Cytoplasm: Light Pink
Collagen: Pink
Muscle: Pink/Rose
Erythrocytes: Pink/Red
Nuclei: Blue
Control Tissue: Any well fixed paraffin embedded or frozen tissue section. Cell smear.
H&E Staining Kit (Hematoxylin and Eosin) protocol summary
Deparaffinize and hydrate.
Apply adequate Hematoxylin and incubate for 5 minutes.
Apply adequate Bluing Reagent and incubate for 10-15 seconds.
Apply adequate Eosin Y Solution (Modified Alcoholic) and incubate for 2-3 minutes.
Clear and mount.
Other Notes
Please note, the 500 mL selling size, was previously described as 1 Kit. There has been no change to the physical product.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient