Abcam, ab246530, Human DMP1 ELISA Kit

Size: 1 x 96Tests
Human DMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DMP1 with a sensitivity of 22.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 22.58 pg/mL,
Range:171.875 - 11000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human DMP1 ELISA Kit (ab246530) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DMP1 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DMP1 with 22.58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein DMP1 also known as Dentin Matrix Protein 1 is part of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family and plays an important role in bone and tooth mineralization. It is encoded by the DMP1 gene and has a molecular weight of around 52 kDa. DMP1 localizes mainly to the extracellular matrix of bone and dentin and is highly expressed in osteoblasts odontoblasts and hypertrophic chondrocytes. This protein regulates the formation of hydroxyapatite a critical mineral component in the formation of hard tissues.
Biological function summary
The protein DMP1 is essential for phosphate homeostasis skeletal development and mineralization processes. It acts as a nucleator in the deposition of calcium phosphate minerals. As part of a protein complex DMP1 interacts with other extracellular matrix proteins influencing their functions. DMP1 plays an important role in the maturation of bone matrix and dentin effectively mediating the transformation of mesenchymal stem cells into osteoblasts and odontoblasts. It is also implicated in the regulation of gene expression linked to mineralization.
Pathways
DMP1 is closely involved in the bone mineralization and FGF (Fibroblast Growth Factor) signaling pathways. These pathways are important for maintaining bone density and controlling phosphate metabolism. DMP1 interacts with other proteins like PHEX (Phosphate Regulating Endopeptidase Homolog X-Linked) and MEPE (Matrix Extracellular Phosphoglycoprotein) within these pathways to facilitate proper mineralization of bone and teeth. Furthermore DMP1 functions alongside other SIBLING family proteins to modulate mineralization through their collective roles in the matrix.
DMP1 mutations or dysfunctions lead to conditions such as autosomal recessive hypophosphatemic rickets and osteomalacia. These conditions result from defective phosphate metabolism affecting skeletal mineralization and causing brittle bones and dental anomalies. In these scenarios DMP1's relationship with PHEX becomes evident as both proteins work together to regulate phosphate levels. Disruption in their function can significantly alter mineralization balance highlighting the necessity of DMP1 in maintaining skeletal and dental health.