Abcam, ab246825, Eosin Y Solution (Aqueous)

Size: 500mL
Eosin Y solution ( Aqueous)( ab246825) is a ready-to-use histology and cytology reagent for cytoplasmic staining and is commonly used as a counterstain for Hematoxylin. - Suitable with paraffin embedded or frozen tissue sections - Compatible with our Lab essentials imaging workflow offering
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 4 - 6Constituents: 1% Eosin Y

Product details:
Eosin Y Solution (Aqueous) ab246825 is intended for use in the histological demonstration of cytoplasm and is commonly used as a counterstain for Hematoxylin. When used correctly, various shades of pink can be obtained to aid in visualization of tissue components. Erythrocytes, collagen, and the cytoplasm of muscle or epithelial cells will stain with different shads of pink.
Cytoplasm: Pink to Red.
Erythrocytes Pink to Red.
Nuclei: Black/Blue (Hematoxylin).
Conterstain:
Any fixed or frozen tissue. Cell smear.
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. If sections are Zenker-fixed, remove mercuric chloride crystals using iodine and clear with sodium thiosulfate. Rinse in running tap water.
3. Rinse slide in distilled water.
4. Stain slide in Hematoxylin, Mayer's for 5 minutes.
5. Rinse slide in running tap water for 2-3 minutes.
6. Apply Bluing Reagent for 30 seconds.
7. Rinse in distilled water.
8. Stain slide in Eosin Y Solution for 5 minutes.
9. Rinse quickly in 95% alcohol followed by 2 minutes in absolute alcohol.
10. Clear, and mount in synthetic resin.
Other products for staining tissue sections
Find more kits and reagents in the
special stains guide
, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient