Abcam, ab247286, Anti-IRS1+IRS2 antibody [EP263Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal IRS1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP263Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen used for this product shares 78% homology with IRS2. Cross-reactivity with this protein has not been confirmed experimentally.

Product details:
ab247286 is the carrier-free version of
ab40777
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The targets IRS1 and IRS2 also known as Insulin Receptor Substrate 1 and 2 are important adaptor proteins that play an important role in insulin signaling. IRS1 and IRS2 have molecular masses of approximately 180 kDa and 185 kDa respectively. These proteins are expressed in a wide range of tissues but show particularly high expression in insulin-responsive tissues such as muscle liver and adipose tissues. IRS1 and IRS2 function by interacting with activated insulin receptors allowing subsequent recruitment of downstream signaling molecules.
Biological function summary
IRS1 and IRS2 translate insulin receptor activation into metabolic and mitogenic effects. These proteins form signaling complexes that mediate the action of insulin and insulin-like growth factor 1 (IGF-1). IRS1 and IRS2 interact with several signaling molecules including PI3K and GRB2 which are essential for the activation of downstream pathways. Their role is essential in glucose homeostasis cell growth and differentiation. In some contexts IRS1 and IRS2 also participate in signaling pathways influenced by other growth factors.
Pathways
IRS1 and IRS2 have pivotal roles in the insulin signaling and IGF-1 signaling pathways. They facilitate signal transduction from the insulin and IGF-1 receptors impacting both metabolic and growth-related pathways. Within these pathways their interaction with PI3K highlights their importance in glucose uptake and utilization while their connection with SHC and ERK pathways emphasizes their involvement in cell growth and proliferation. The coordinated action of IRS1 and IRS2 helps maintain energy balance and cellular functions.
IRS1 and IRS2 have significant associations with type 2 diabetes and certain cancers. In type 2 diabetes alterations in IRS1 and IRS2 signaling can impair glucose metabolism leading to insulin resistance. Genetic variations or dysregulation in their function often contribute to diabetes susceptibility. Additionally IRS1 and IRS2 are linked to cancer as their pathways overlap with pathways that influence cell growth and survival. Increased signaling can enhance cell proliferation and resistance to apoptosis with relevant proteins like PI3K and AKT further involved in these processes.