Abcam, ab247510, Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NDUFAF1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2796,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247510 is the carrier-free version of
ab79826
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFAF1 also known as NADH dehydrogenase (ubiquinone) complex I assembly factor 1 plays an essential role in the mitochondrial respiratory chain by assisting the assembly of complex I. This protein has a mass of approximately 36 kDa. NDUFAF1 expression occurs mainly in tissues with high metabolic activity like the brain heart and skeletal muscle. It facilitates the proper formation and function of the complex I assembly allowing efficient electron transport essential for cellular respiration.
Biological function summary
NDUFAF1 is a critical assembly factor integral to forming the mitochondrial complex I also known as NADH:ubiquinone oxidoreductase. NDUFAF1 contributes to the stability and proper function of this multi-component enzyme system which is important in cellular energy production. As part of the complex it ensures efficient ATP generation through oxidative phosphorylation which is the primary source of energy in aerobic eukaryotic cells.
Pathways
NDUFAF1 functions predominantly in the oxidative phosphorylation pathway. This involvement connects NDUFAF1 to the fundamental molecular processes sustaining life. The protein plays a role alongside others like NDUFV1 and NDUFS1 in maintaining complex I activity. These relationships ensure the effective transfer of electrons from NADH to ubiquinone promoting efficient energy production throughout the cell.
NDUFAF1 mutations or dysfunction have been linked to mitochondrial disorders such as Leigh syndrome and complex I deficiency. These conditions are often characterized by a disruption in mitochondrial energy metabolism leading to severe neurological and muscular symptoms. Furthermore NDUFAF1 dysfunction can indirectly affect other proteins like NDUFS4 which is also involved in complex I assembly and function exacerbating the disease phenotypes. Mitochondrial diseases often arise from impaired pathways involving several network proteins highlighting the significance of proper NDUFAF1 function.