Abcam, ab247694, Anti-ENTPD5 antibody [EPR3784] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ENTPD5 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3784,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247694 is the carrier-free version of
ab108603
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ENTPD5 also known as ectonucleoside triphosphate diphosphohydrolase 5 or NTPDase-5 primarily acts as an ER-resident glycoprotein that hydrolyzes nucleoside diphosphates into monophosphates. This enzyme weighs approximately 45 kDa. It is predominantly expressed in liver and pancreas tissues. Functionally ENTPD5 regulates cellular levels of UDP by converting GDP and its role in the re-glycosylation of proteins.
Biological function summary
This enzyme plays a role in protein folding and quality control within the endoplasmic reticulum. ENTPD5 influences the ER-associated degradation (ERAD) pathway which deals with the removal of misfolded proteins. The enzyme does interact with other proteins and complexes including calnexin and calreticulin chaperone systems suggesting its integration in glycan-processing machinery.
Pathways
ENTPD5 integrates into the protein glycosylation and turnover pathways. It links with the AKT/mTOR signaling pathway by regulating the availability of UDP-GlcNAc a substrate for N-glycosylation of proteins. ENTPD5 relates to proteins like AKT and mTOR that are involved in protein synthesis cell growth and survival signaling pathways.
ENTPD5 has an association with cancer notably prostate cancer. The enzyme modulates the Warburg effect linked to the increased reglycosylation of proteins promoting tumor progression. It also connects to diabetes through its role in insulin signaling pathways where aberrant glycoprotein fragments may affect insulin receptor activity. Here ENTPD5 shows a connection to proteins involved in insulin signaling such as the insulin receptor.