Abcam, ab2477, Anti-PAK1 (phospho T423) + PAK2 (phospho T402) + PAK3 (phospho T436) antibody

Size: 100µg
Rabbit Polyclonal PAK1 phospho T423 antibody. Suitable for Dot, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Serine/threonine-protein kinase PAK 1 phospho T423 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Serine/threonine-protein kinase PAK 1 phospho T423 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q13153,
Specificity:This affinity purified antibody is directed against human PAK (p21-GTPase Activated Protein Kinase). This phospho specific polyclonal antibody is specific for phosphorylated pT423 of Human PAK 1/2/3. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA. Cross reactivity with PAK 1/2/3 from other species has not been determined.

Product details:
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle