Abcam, ab247816, Anti-MCM2 (phospho S108) antibody [EPR4121] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MCM2 phospho S108 antibody. Carrier free. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4121,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247816 is the carrier-free version of
ab109271
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.
Biological function summary
MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.
Pathways
MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.
MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.