Abcam, ab247838, Anti-HUS1 antibody [EPR5132] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HUS1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5132,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247838 is the carrier-free version of
ab109371
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HUS1 also known as checkpoint protein HUS1 homolog is a protein that plays an important role in DNA damage response. It has a molecular mass of about 34 kDa and is expressed in various tissues with notable expression in testis and thymus. HUS1 is part of the Rad9-Rad1-Hus1 (9-1-1) complex which acts as a sensor for DNA damage and activates downstream signaling pathways for DNA repair mechanisms.
Biological function summary
HUS1 is important in maintaining genomic stability and integrity. It forms a complex with RAD9 and RAD1 creating the 9-1-1 complex that localizes to sites of DNA damage. This complex enables the recruitment and activation of several key DNA repair proteins facilitating the repair process. HUS1 plays a role in the activation of cell cycle checkpoints essential for preventing the propagation of damaged DNA during cell division.
Pathways
HUS1 is significantly involved in the DNA damage response and cell cycle regulation pathways. The protein connects into the ATR signaling pathway where it helps activate ATR kinase in response to DNA damage. Related proteins in these pathways include ATM and CHK1 which also serve in coordinating the cellular response to DNA injuries.
HUS1 is associated with cancer pathogenesis particularly in regard to its role in DNA repair. Mutations or dysregulation in HUS1 can lead to genomic instability a hallmark of cancerous cells. In addition to cancer HUS1 plays a role in hereditary diseases such as Ataxia-telangiectasia due to its connection with the ATM protein which is mutated in this disorder. Understanding HUS1's function and interactions might provide insight into therapeutic approaches for these conditions.