Abcam, ab247848, Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AP2M1 phospho T156 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4700,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247848 is the carrier-free version of
ab109397
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein AP2M1 also known as AP-2 complex subunit mu plays an important role in clathrin-mediated endocytosis. It weighs approximately 50 kDa. AP2M1 is mainly expressed in different cell types including neuronal epithelial and fibroblast cells. This protein is part of the adaptor protein (AP) complex family specifically the AP-2 complex which facilitates the recruitment of clathrin and cargo proteins to form clathrin-coated vesicles at the plasma membrane.
Biological function summary
The AP2M1 protein is involved in the sorting and trafficking of membrane proteins and lipids. It functions as part of the AP-2 complex a heterotetrameric assembly composed of two large adaptins a medium chain (mu) adaptin like AP2M1 and a small chain adaptin. This complex helps recognize and bind specific sorting signals found on the cytosolic tails of transmembrane cargo proteins allowing their incorporation into forming vesicles during endocytosis.
Pathways
AP2M1 contributes significantly to the endocytic pathway and the regulation of receptor-mediated signaling. Involvement with the insulin signaling pathway highlights its role in internalizing insulin receptors and modulating insulin sensitivity. AP2M1 interacts with other proteins such as clathrin which influences the assembly of clathrin-coated pits at the cell membrane and also with accessory proteins like dynamin facilitating the fission of vesicles during endocytosis.
Research associates AP2M1 with neurodegenerative diseases such as Alzheimer's disease due to its role in synaptic vesicle recycling and endocytosis affecting neuron function. It also relates to metabolic disorders like diabetes as improper function or regulation of AP2M1 can impact insulin receptor internalization and downstream signaling. Other proteins connected to AP2M1 through these disorders include clathrin and dynamin which are pivotal for maintaining the integrity of cellular transport mechanisms.