Abcam, ab247849, Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Hormone sensitive lipase/HSL phospho S853 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2329(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antibody only detects HSL phosphorylated on serine 853.

Product details:
ab247849 is the carrier-free version of
ab109400
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hormone sensitive lipase (HSL) also known as HSL lipase or HSL is an important enzyme involved in lipid metabolism. This enzyme mechanically functions to hydrolyze stored triglycerides into free fatty acids and glycerol inside adipocytes. It is expressed largely in adipose tissue but is also found in heart muscle and other tissues. The molecular weight of HSL is approximately 83 kDa. Its activity is regulated by hormonal signals which enables its role in lipolysis making it a central player in energy homeostasis.
Biological function summary
Hormone sensitive lipase plays an important role in the mobilization of fatty acids which are used by the body as an energy source. HSL is not part of a larger protein complex but it interacts with various hormones and signaling molecules. The presence of HSL in different tissues allows it to facilitate lipid droplet breakdown proving essential in energy release during fasting and exercise.
Pathways
The activity of hormone sensitive lipase integrates into the lipolytic pathways responsible for breaking down stored lipids. HSL is interconnected notably within the lipid metabolism pathway and related to the insulin signaling pathway. Proteins such as adipose triglyceride lipase (ATGL) work alongside HSL where ATGL initiates the first step of triglyceride hydrolysis after which HSL further processes the diacylglycerides therefore collaborating in lipid degradation and utilization.
Hormone sensitive lipase has associations with conditions like obesity and type 2 diabetes. Dysregulation of HSL activity can lead to improper lipid mobilization and accumulation contributing to metabolic syndrome. In obesity HSL activity decreases affecting how the body manages fat stores. In these contexts proteins like perilipin which regulates lipase access to lipid droplets play a role by affecting HSL's efficiency and functional capacity in energy management and lipid metabolism.