Abcam, ab247852, Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PI4 kinase beta/PI4KB antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3758(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247852 is the carrier-free version of
ab109418
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI4 kinase beta also known as PI4KB is an important enzyme involved in lipid signaling. It catalyzes the phosphorylation of phosphatidylinositol (PI) to produce phosphatidylinositol 4-phosphate (PI4P) an essential lipid for various cellular processes. PI4KB has a molecular mass of approximately 92 kDa. This enzyme shows expression in various tissues including the brain liver and pancreas. In cells PI4KB predominantly localizes at the Golgi apparatus and some plasma membrane sites.
Biological function summary
The enzyme plays a role in maintaining proper intracellular membrane structure and function. It operates as part of a larger complex involving additional elements such as the small GTPase ARF1 and the scaffold protein ACBD3 which are necessary for its recruitment and activity at Golgi membranes. PI4KB facilitates vesicular trafficking and supplies substrates for the production of other phosphoinositides impacting diverse cellular events like cell growth and signal transduction.
Pathways
PI4KB is an important participant in the phosphoinositide signaling pathway. This pathway regulates membrane dynamics and trafficking influencing cell signaling and membrane identity. PI4KB interacts with proteins such as PI4K2A and PI4K2B in the synthesis of different phosphoinositides which are components of the signaling cascade that include PI3K/AKT pathway having implications in cell growth and survival.
PI4KB has associations with certain infectious diseases and neurological disorders. The enzyme is linked to hepatitis C virus (HCV) replication where it interacts with viral proteins like NS5A facilitating the formation of replication complexes. Furthermore PI4KB has links to neurodegenerative disorders like Alzheimer's disease possibly through its influence on vesicular trafficking and lipid metabolism. These associations highlight its importance in disease mechanisms and as a potential therapeutic target.