Abcam, ab2480, Anti-MYL12A (phospho S19) antibody

Size: 50µg
Anti-MYL12A (phospho S19) antibody (ab2480) is a rabbit polyclonal antibody detecting MYL12A in Western Blot, IP, IHC-P, ICC/IF, ELISA . Suitable for Caenorhabditis elegans, Human, Mouse, Rat, . - Over 140 publications - Trusted since 2002
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Caenorhabditis elegans, Rat,
Applications:ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MYL12A phospho S19 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P19105,
Specificity:ab2480 can detect the phosphorylated serine residue in both the human and mouse protein. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.This phospho specific polyclonal antibody is specific for phosphorylated pS19 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.Customers have seen variable performance with Xenopus laevis.

Product details:
What is this antibody validated in?
Anti-MYL12A (phospho S19) antibody (ab2480) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Caenorhabditis elegans, Human, Mouse, Rat, samples.
What is the molecular weight of MYL12A?
Anti-MYL12A (phospho S19) (ab2480) specifically detects a band for MYL12A (UniProt: P19105) at a molecular weight of 20kDa.
Trusted by the scientific community
Anti-MYL12A (phospho S19) (ab2480) was first used in a scientific publication in 2002 and has been cited over 140 times in peer-reviewed journals.
Reviewed by scientists
Anti-MYL12A (phospho S19) (ab2480) has over 15 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle