Abcam, ab248166, Anti-RRP42 antibody [EPR7452] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RRP42 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7452,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248166 is the carrier-free version of
ab126750
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RRP42 also known as EXOSC7 is a component of the RNA exosome complex. It weighs around 28 kDa. This protein is found mainly in the nucleolus and cytoplasm where it helps with RNA processing and degradation. RRP42 acts as a scaffold within the exosome complex playing a role in structuring the exosome for interactions with other proteins and RNA substrates.
Biological function summary
RRP42 forms part of the core of the exosome complex which is essential for maintaining RNA homeostasis. This exosome complex includes several other proteins like RRP4 RRP40 and EXOSC10 creating a multiprotein assembly that processes a variety of RNA species. Through these interactions RRP42 participates in the degradation and maturation of different types of RNA like ribosomal RNA messenger RNA and small nuclear RNA.
Pathways
RRP42's role in the exosome relates to RNA metabolic processes and related pathways. It engages in the RNA processing pathway where it works on properly modifying RNA molecules. Another pathway where RRP42 is active is the surveillance pathway that protects the cell from faulty RNA. In these roles it associates with other proteins such as SKIV2L and DIS3 which also contribute to RNA turnover and quality control.
The activity of RRP42 influences conditions related to RNA processing defects. Improper function or regulation of RRP42 may associate with specific genetic disorders like pontocerebellar hypoplasia and some cancers where RNA metabolism becomes disrupted. Connections to these diseases often involve interactions with proteins like EXOSC3 and EXOSC8 which are part of the same exosome complex and contribute to the disease phenotypes through similar mechanisms.