Abcam, ab248192, Anti-POLG antibody [EPR7295] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal POLG antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7295,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248192 is the carrier-free version of
ab128862
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
POLG also known as DNA polymerase gamma is an enzyme essential for mitochondrial DNA replication and repair. It is composed of a catalytic subunit with approximately 140 kDa mass. This protein is mainly expressed in tissues with high energy demands such as liver heart and skeletal muscles. POLG functions by synthesizing the leading and lagging strands during mitochondrial DNA replication ensuring mitochondrial DNA integrity.
Biological function summary
DNA polymerase gamma plays an important role in maintaining mitochondrial genomes. This protein operates within a complex called the POLG complex which is vital for mitochondrial DNA replication machinery. The catalytic subunit works alongside two accessory subunits that enhance its processivity and accuracy during DNA synthesis. Mutations in POLG can impair mitochondrial DNA replication leading to mitochondrial dysfunction.
Pathways
POLG is integral in the mitochondrial DNA replication and repair pathways. It is closely linked with the pathways ensuring mitochondrial gene expression and energy production. POLG acts in concert with mitochondrial DNA helicase TWINKLE and mitochondrial single-stranded DNA binding protein (mtSSB) both contributing to the replication fork activity and stability. POLG's role ensures proper mitochondrial function and consequently cellular energy homeostasis.
POLG mutations have been associated with diverse mitochondrial disorders including Alpers-Huttenlocher syndrome and progressive external ophthalmoplegia. POLG-related disorders often result from impaired mitochondrial DNA replication leading to energy deficits in cells. In particular mutations affecting the POLG complex's performance can influence the onset of these conditions sometimes involving interactions with other proteins like TWINKLE and mtSSB which further complicate the mitochondrial dysfunction seen in such diseases.