Abcam, ab248288, Anti-PERP antibody [EPR7885] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PERP antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7885,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248288 is the carrier-free version of
ab129083
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PERP also known as P53 apoptosis effector related to PMP-22 is a protein with an approximate mass of 23 kDa. It mainly operates as an effector in the p53-dependent apoptosis pathway. PERP is known for facilitating cell-cell adhesion as a part of the desmosome complex. Researchers have found its expression primarily in epithelial tissues where it contributes to maintaining cell structure integrity. PERP's role is significant in tissues experiencing stress where it upregulates in response to p53 activation.
Biological function summary
PERP plays an active role in cellular processes like adhesion and apoptosis. As a part of the desmosome it helps in connecting epithelial cells. This function is important for preserving tissue architecture and responding to mechanical stress. PERP's involvement in apoptosis arises from its interactions in the p53 pathway supporting the process when cells require programmed death. These roles are essential for maintaining balanced cell proliferation and death key for tissue homeostasis.
Pathways
PERP is closely tied to the p53 pathway and the desmosomal adhesion process. The p53 pathway involves one of the primary tumor suppressor proteins and regulates the cell cycle while the desmosomal adhesion pathway maintains the structural cohesion between cells. PERP acts in concert with various proteins such as JUP (Plakoglobin) strengthening cellular adhesions and responding to apoptotic signals. This connectivity allows PERP to perform dual roles in maintaining cell stability and facilitating apoptosis when necessary.
PERP has associations with squamous cell carcinoma and epidermolysis bullosa. Disruption of PERP functions can lead to improper apoptosis contributing to cancer progression. In the case of epidermolysis bullosa alterations in desmosomal structure connected to PERP can weaken skin integrity leading to blistering. In these conditions proteins like JUP work alongside PERP highlighting the importance of balance between cell adhesion and controlled cell death in disease prevention.