Abcam, ab248289, Anti-RACK1 antibody [EPR7388] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RACK1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7388,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human, Mouse,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248289 is the carrier-free version of
ab129084
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RACK1 or Receptor for Activated C Kinase 1 is an approximately 36 kDa protein that acts as scaffolding facilitating protein-protein interactions. It has a conserved WD40 repeat domain structure essential for its functions. RACK1 is ubiquitously expressed in various tissues including brain liver and lungs. It aids in assembling signaling complexes by anchoring signaling proteins contributing to various cellular responses.
Biological function summary
The function of RACK1 influences numerous cellular processes such as cell growth migration and apoptosis. It forms part of the ribosome serving a function in translation regulation. RACK1 participates in G-protein mediated signaling as well and binds to activated PKC (protein kinase C) isoforms promoting effective signaling pathways that affect cell behavior.
Pathways
Studies identify RACK1 within the MAPK and JAK/STAT pathways. In the MAPK pathway RACK1 works alongside proteins like ERK1/2 to mediate cellular responses to external stimuli. Within the JAK/STAT pathway its interaction with kinases influences the transcriptional activation of genes involved in immune responses and cell growth signaling emphasizing its role in regulating important cellular activities.
Researchers have linked RACK1 to certain cancers and neurodegenerative diseases. In cancer RACK1's interaction with PKC and other signaling proteins like Src can affect cell proliferation and angiogenesis features significant in tumor progression. Regarding neurodegenerative diseases RACK1's involvement in amyloid precursor protein processing connects it to Alzheimer's disease progression with potential interactions with proteins like presenilin highlighting its role in pathophysiology.