Abcam, ab248352, Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LMO4 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6731(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248352 is the carrier-free version of
ab131030
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LIM-only protein 4 (LMO4) is a transcriptional regulator distinguished by its two LIM domains. It has an estimated molecular weight of approximately 22 kDa. LMO4 is expressed in several tissues but is notably present in the nervous system particularly the developing brain and spinal cord. The protein is also referred to as LMO4a in some contexts and plays a significant role in cellular processes by modulating gene expression.
Biological function summary
LMO4 participates in various developmental processes and acts as a cofactor within transcriptional complexes. It interacts with other proteins to regulate the transcription of genes critical for neural development and differentiation. LMO4 does not function in isolation but as part of larger multiprotein complexes influencing various signaling pathways linked to cell growth and differentiation.
Pathways
The LMO4 protein has strong connections to key signaling pathways including the Notch and Wnt pathways. These pathways are essential in neurogenesis and cellular differentiation processes. LMO4 interacts with proteins such as GATA3 and neurogenin acting as an intermediary that influences the transcriptional activity required for cellular development.
Scientists have linked alterations in LMO4 expression to conditions like breast cancer and neurodevelopmental disorders. Abnormal levels of LMO4 expression can disrupt cellular proliferation and differentiation contributing to pathogenesis. In breast cancer LMO4 interacts with the protein estrogen receptor promoting tumor growth and malignancy. Understanding these interactions helps unravel the molecular mechanisms underlying these diseases and offers targets for therapeutic intervention.