Abcam, ab248394, Anti-ARF6 antibody [EPR8357] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ARF6 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8357,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248394 is the carrier-free version of
ab131261
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ARF6 protein also known as ADP-ribosylation factor 6 is a small GTPase with a molecular weight of approximately 21 kDa. The protein belongs to the ARF family which plays roles in regulating vesicular trafficking and cytoskeletal dynamics. ARF6 is ubiquitously expressed across many tissues most abundantly in the brain muscle and epithelial cells. Its activity depends on cycling between an inactive GDP-bound form and an active GTP-bound form which is important for its function in cellular processes.
Biological function summary
The activity of ARF6 influences membrane trafficking and actin cytoskeleton remodeling. The protein helps mediate endocytosis and exocytosis as well as modulating cell adhesion and migration. ARF6 interacts with various effector proteins to form a complex that is involved in the coordination of these processes. This complex activity underlines ARF6's roles in cell signaling and intracellular trafficking mechanisms.
Pathways
ARF6 plays key roles in the Wnt signaling pathway and the E-cadherin recycling pathway. In the Wnt pathway ARF6 regulates the internalization of receptors and affects downstream signaling. In E-cadherin recycling ARF6 orchestrates trafficking events that ensure proper cell-cell adhesion. These functions often involve interactions with related proteins like ARF1 and Rac1 which further modulate ARF6's involvement in these signaling events influencing cellular behavior.
ARF6 has a connection to certain cancers and neurological conditions. Its dysregulation can lead to increased metastasis in cancers partly due to altered cell motility and invasion linked to changes in cell adhesion. Additionally the misregulation of ARF6 is implicated in Alzheimer's disease where it may disrupt neuronal trafficking and contribute to synaptic dysfunction. These disease associations also involve related proteins such as EFA6 and ARHGAP10 which can modulate ARF6 activity and its effects on pathological processes.