Abcam, ab248483, Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Aldolase B antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3137,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248483 is the carrier-free version of
ab133333
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aldolase B and Aldolase C are enzymes playing key roles in breaking down fructose and glucose in glycolysis and gluconeogenesis. Aldolase B also known as fructose-bisphosphate aldolase B has a mass of approximately 39 kDa and is mainly found in the liver kidney and small intestine. Aldolase C with a similar mass to Aldolase B is primarily expressed in the brain and nervous tissues. These enzymes are part of the aldolase family which catalyzes the conversion of fructose-16-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate.
Biological function summary
Both Aldolase B and C participate in key metabolic processes. Aldolase B is essential for fructose metabolism and degradation being especially important in tissues where fructose is a main sugar source. It is not part of a larger protein complex. Aldolase C on the other hand is significant in energy production in neurons contributing to overall brain metabolism. Both enzymes contribute to the conversion of sugars into energy necessary for cellular functions.
Pathways
Aldolase enzymes are integral to the glycolysis and gluconeogenesis pathways. In glycolysis they participate in the step where fructose-16-bisphosphate splits into two trioses facilitating energy release from glucose. In gluconeogenesis the reverse occurs assisting in the production of glucose. These pathways are important for maintaining energy balance in cells. Aldolase B notably interacts with fructokinase which phosphorylates fructose to fructose-1-phosphate the substrate for aldolase action.
Deficiencies or mutations in Aldolase B are linked to hereditary fructose intolerance (HFI) a disorder where fructose ingestion leads to toxic accumulation within cells and severe symptoms. Both Aldolase B and C can also be markers in conditions like hepatocellular carcinoma and neurodegenerative disorders. In HFI the deficiency in Aldolase B function leads to accumulations affecting liver kidney and digestive tissues while disturbances in Aldolase C may impact neurological health.