Abcam, ab248601, Anti-Human IgA antibody [EPSISR10] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal IGHA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPSISR10,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for the heavy chain of IgA.

Product details:
ab248601 is the carrier-free version of
ab133660
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Human IgA also known as Immunoglobulin A is an antibody that plays a critical role in the immune system. It weighs approximately 160 kDa and is predominantly found in mucosal areas such as the respiratory and gastrointestinal tracts as well as in saliva tears and breast milk. In its molecular form IgA often exists as a dimer two IgA molecules linked by a joining (J) chain. The primary function of IgA is to protect mucosal surfaces by neutralizing pathogens and toxins preventing them from adhering to epithelial cells.
Biological function summary
IgA contributes to the immune defense by participating in immune exclusion mechanisms. As part of this process IgA interacts with the polymeric immunoglobulin receptor (pIgR) to transport across epithelial cells making it important for mucosal immunity. IgA is also connected with secretory components that further stabilize and enhance its longevity in mucosal secretions. It plays an integral role in forming complexes that assist in eliminating antigens from mucosal surfaces.
Pathways
Human IgA is an important player in the mucosal immune response pathway defending against pathogens by zeroing in on antigen neutralization. Its interactions with the pIgR are central to the transport mechanism through epithelial cells where the IgA-pIgR complex becomes the secretory IgA. This pathway shares a strong connection with related proteins such as IgM another immunoglobulin involved in the early stages of immune response and found in serum.
IgA is associated with IgA nephropathy a kidney disorder caused by the deposition of IgA in the kidneys leading to inflammation and functional impairment. Additionally IgA plays a role in celiac disease where IgA antibodies are produced in response to gluten and can contribute to intestinal damage. In these conditions the relationship between IgA and other proteins like complement component 3 (C3) is significant as improper regulation of these pathways may lead to tissue damage and disease progression.