Abcam, ab248622, Anti-Bub3 antibody [EPR5319(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal BUB3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5319(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248622 is the carrier-free version of
ab133699
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bub3 also known as budding uninhibited by benzimidazoles-3 is an important component of the spindle checkpoint machinery. Weighing approximately 35 kDa this protein is expressed ubiquitously in eukaryotic cells. Bub3 interacts with other spindle checkpoint proteins and takes part in chromosome segregation during mitosis. It primarily functions in the nucleus during the cell cycle ensuring proper attachment of chromosomes to the mitotic spindle which is important for accurate cell division.
Biological function summary
Bub3 participates in the mitotic checkpoint complex an essential mediator of cell cycle arrest in response to improper spindle attachment. This complex includes Bub1 BubR1 and Mad3 where Bub3 acts as an adapter facilitating interactions within the complex. It helps maintain chromosomal stability by preventing premature anaphase onset therefore ensuring that damaged or unaligned chromosomes do not progress through cell division. By doing so Bub3 safeguards against aneuploidy and genomic instability.
Pathways
Bub3 is linked to the spindle assembly checkpoint (SAC) and the cell cycle regulation pathway. In SAC Bub3 collaborates closely with proteins such as Bub1 and Mad1 to inhibit the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes are properly aligned. The interplay between Bub3 and BubR1 is important for delaying the chief exit phases of mitosis ensuring that all cellular conditions are favorable for division. These interactions prevent errors that might cause irreparable damage or cell death.
Bub3 has associations with cancer and some neurodegenerative diseases. Its dysregulation can lead to aneuploidy a hallmark of many cancers through faulty chromosome segregation. In several cancer types altered expression of Bub3 and its partners such as Bub1 and Mad2 has been documented. Similarly emerging studies suggest links between Bub3 malfunction and neurodegeneration where alterations in cell division and genomic stability may contribute to disease progression.