Abcam, ab248826, Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal IMPDH1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9679(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248826 is the carrier-free version of
ab137112
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The proteins IMPDH2 and IMPDH1 also known as inosine-5'-monophosphate dehydrogenase 2 and 1 are enzymes involved in purine metabolism. IMPDH1 has a mass of approximately 55 kDa while IMPDH2 measures around 56 kDa. Both proteins catalyze the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) an essential step in the de novo synthesis of guanine nucleotides. They are expressed in various tissues with highest levels found in blood and immune system cells reflecting their important role in cell proliferation.
Biological function summary
These enzymes perform critical functions by facilitating the synthesis of guanine nucleotides which are building blocks of DNA and RNA. IMPDH1 and IMPDH2 do not function within large multimeric complexes but exhibit regulatory roles over cellular proliferation and differentiation. The balance between these enzymes impacts the overall guanine nucleotide pool affecting cellular processes such as growth and division.
Pathways
These enzymes link to nucleotide biosynthesis pathways particularly the guanine nucleotide synthesis pathway. This pathway integrates with the purine metabolism network. IMPDH proteins interact with key enzymes like GMP synthase which directly follows IMPDHs in nucleotide synthesis. Their activity regulates cell proliferative capacity making them integral to processes like DNA replication and repair.
The proteins play significant roles in conditions like cancer and retinitis pigmentosa. IMPDH2 is often overexpressed in rapidly proliferating cancer cells linking it to tumor growth and progression. Meanwhile mutations in IMPDH1 have associations with retinitis pigmentosa a genetic disorder causing retinal degeneration. These enzymes’ dysfunction relates to proteins they interact with in these diseases such as DNA repair proteins in cancer and photoreceptor proteins in retinal disorders.