Abcam, ab248981, Anti-ITPA antibody [EPR8779] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ITPA antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8779,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248981 is the carrier-free version of
ab150420
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ITPA also known as inosine triphosphate pyrophosphatase is an important enzyme responsible for catalyzing the hydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP). This enzyme prevents the accumulation of non-canonical nucleotides in the cell. ITPA has a mass of approximately 21 kDa and is expressed ubiquitously in various tissues indicating its importance across different cell types. The enzyme is also present in HEK 293T cells as researchers often use these cells for studies due to their growth characteristics and transfection efficiency.
Biological function summary
The enzyme plays a pivotal role in nucleotide metabolism by regulating the levels of ITP deoxyinosine triphosphate (dITP) and xanthosine triphosphate (XTP). By hydrolyzing these non-standard nucleotides ITPA maintains genomic integrity and prevents mutations during DNA replication. It is not typically considered part of a larger complex but acts independently to safeguard the nucleotide pool from potentially harmful nucleotide analogs.
Pathways
ITPA's activity is essential in the purine metabolism pathway where it helps maintain a proper balance of nucleotide synthesis and degradation. This function helps prevent the incorporation of non-standard bases into DNA and RNA which can lead to genetic instability. ITPA activity is also intertwined with the salvage pathway of purine nucleotides. This connection highlights its indirect relationship with proteins involved in nucleotide synthesis and repair such as DNA polymerases and other nucleotide-metabolizing enzymes.
ITPA has associations with certain pathological conditions. For example ITPA deficiency can lead to inosine triphosphate accumulation which might contribute to hemolytic anemia. Mutations in the ITPA gene can also impact drug metabolism notably in patients receiving thiopurine drugs such as those used for leukemia treatment. These mutations may lead to adverse drug reactions due to the altered nucleotide balance. The connection with these conditions emphasizes the role of ITPA in maintaining cellular homeostasis and its potential influence on pharmacogenomics.