Abcam, ab249067, Anti-HOOK1 antibody [EPR10102] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HOOK1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10102,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249067 is the carrier-free version of
ab151756
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HOOK1 protein also known as Hklp2 plays important role in cellular processes related to intracellular trafficking. Mechanically HOOK1 functions as a microtubule-binding protein that facilitates the movement of cytoplasmic vesicles. It weighs approximately 88 kDa. It is expressed in various tissues with significant expression observed in the brain and testis. In these tissues HOOK1 assists in ensuring proper distribution and transport of cellular components.
Biological function summary
HOOK1 plays a significant role in cellular organization and is part of the microtubule-associated HOOK family. This family participates in maintaining correct positioning of organelles which is critical for cell division and differentiation. HOOK1 associates with dynein/dynactin motor complexes modulating cytoskeletal dynamics. Through this complex it influences processes such as mitosis and meiosis allowing cells to maintain spatial organization of important structures.
Pathways
HOOK1 is actively involved in intracellular transport pathways and contributes to endocytic and secretory pathways. These include endosome positioning and cargo trafficking within cells. HOOK1 interacts with other proteins such as dynactin in these pathways facilitating transport efficiency and specificity. It also relates closely to proteins involved in lysosome positioning and function within the same pathways ensuring cellular homeostasis is maintained.
Malfunction or dysregulation of HOOK1 has been implicated in male infertility and neurological disorders. Mutations affecting HOOK1 can disrupt spermiogenesis leading to issues such as oligozoospermia a condition characterized by low sperm count. Additionally improper functioning of HOOK1 may contribute to neurodegenerative conditions through dysregulated cellular transport mechanisms in neurons. In both contexts related proteins such as dynactin play cooperative roles and may also be implicated depending on HOOK1 activity.