Abcam, ab249147, Anti-AGO3 antibody [EPR9576] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AGO3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9576,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249147 is the carrier-free version of
ab154844
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AGO3 also known as Argonaute protein 3 is an important component of the RNA-induced silencing complex (RISC). It is part of the Argonaute family which plays a role in RNA silencing processes. AGO3 usually has a molecular mass of around 97 kDa. It is mainly expressed in human tissues such as testes and is responsible for processing small non-coding RNAs including miRNAs and siRNAs. These small RNAs guide AGO3 to complementary RNA sequences facilitating the cleavage and degradation of target mRNA.
Biological function summary
AGO3 contributes to gene expression regulation by silencing specific RNA transcripts. This protein forms a complex with other proteins and RNA molecules to execute its functions. Part of the miRNA and RNA interference pathways AGO3 associates with Dicer and other Argonaute proteins creating an effective silencing machinery that modulates various cellular processes. Its activity is essential for maintaining cellular homeostasis through RNA interference-mediated gene silencing.
Pathways
AGO3 plays a significant role in the RNA interference (RNAi) and the microRNA (miRNA) pathways. These pathways are key in post-transcriptional gene regulation controlling gene expression patterns important for development and differentiation. AGO3 interacts with Dicer a pivotal enzyme in the RNAi pathway facilitating the processing of precursor miRNAs into mature miRNAs further guiding RISC to its target mRNA sequences. These interactions highlight AGO3's critical place in RNA silencing networks.
AGO3 has links to certain cancers and hereditary disorders. Aberrations in its expression or function can impact miRNA-mediated regulation contributing to tumorigenesis and disorders related to gene expression imbalances. For instance AGO3's dysfunction can relate to testicular cancer due to its significant expression in testicular tissue. Connections between AGO3 and other Argonaute family proteins like AGO1 and AGO2 may influence disease development highlighting its importance in understanding disease mechanisms.