Abcam, ab249156, Anti-TCEB2/Elongin-B antibody [EPR10441(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TCEB2/Elongin-B antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10441(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249156 is the carrier-free version of
ab154854
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TCEB2 also known as Elongin-B is a protein that is part of the Elongin complex. It has an approximate molecular mass of 14 kDa. Elongin-B is widely expressed in various tissues including the heart liver and kidney. This protein works mechanically by increasing the transcription elongation efficiency of RNA polymerase II which is key for proper gene expression. Elongin-B functions within a cellular environment where it modulates transcriptional processes.
Biological function summary
Elongin-B participates as a component of the Elongin complex which also includes Elongin-C and Elongin-A. This complex enhances the rate of transcriptional elongation by alleviating the effects of transcriptional pausing or stalling. By facilitating the transcription of DNA to mRNA Elongin-B indirectly influences various cellular functions and growth processes. It acts by stabilizing other subunits of the complex contributing to the overall efficiency of gene expression systems.
Pathways
Elongin-B takes part in the ubiquitin-proteasome pathway a critical cellular system for protein degradation and turnover. It partners with others like Von Hippel-Lindau (VHL) protein within this pathway. Apart from protein degradation Elongin-B is also involved in the regulation of hypoxia-inducible factor (HIF) connecting it to pathways related to cellular response to oxygen levels. This interaction illustrates the protein's role in managing levels of various proteins related to stress responses in cells.
Elongin-B has implications in cancer and von Hippel-Lindau disease. Abnormal functioning or expression influences cellular mechanisms like uncontrolled cell growth seen in cancer proliferation. Elongin-B's interaction with VHL protein affects von Hippel-Lindau syndrome a condition that leads to tumor and cyst development in different parts of the body. The study of Elongin-B's role in these pathologies helps in understanding disease progression and identifying potential therapeutic targets.