Abcam, ab249179, Anti-PHOX2A antibody [EPR9071] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PHOX2A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9071,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249179 is the carrier-free version of
ab155084
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHOX2A also known as Arix among other names is a transcription factor with an approximate mass of 34 kDa. It plays an essential role in the development of the autonomic nervous system. PHOX2A shows specific expression in neuronal tissues particularly in the noradrenergic and cholinergic neurons. Its function involves the regulation of genes that control neuronal differentiation and development.
Biological function summary
PHOX2A is key in the regulation of noradrenergic phenotype important for the formation and maintenance of the autonomic nervous system. It interacts as part of a transcriptional complex working directly to activate the expression of genes necessary for the synthesis of catecholamines such as dopamine beta-hydroxylase. The protein ensures the proper differentiation of neural crest cells into sympathetic and parasympathetic neurons by influencing its genetic targets.
Pathways
PHOX2A is integral to the development of the autonomic nervous system pathway and the noradrenergic biosynthesis pathway. Much of its function is orchestrated through interactions with other transcription factors like PHOX2B. By regulating gene expression within these pathways PHOX2A contributes to the broader network of neural crest cell development impacting cellular processes that underpin nervous system formation and function.
Mutations in PHOX2A have been linked to congenital central hypoventilation syndrome and Hirschsprung disease. In these conditions PHOX2A's relationships with other proteins notably PHOX2B are significant as these proteins share overlapping roles in neural development. Disruptions in PHOX2A consequently impair pathways and processes critical for autonomic control and gut innervation leading to the aforementioned disorders.