Abcam, ab249262, Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Dynein intermediate chain 1/DNAI1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11222,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249262 is the carrier-free version of
ab156567
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dynein intermediate chain 1 also known as DNAI1 is a component of the cytoplasmic dynein complex. Mechanically this protein serves as an adaptor or linker within the larger dynein motor protein complex essential for intracellular transport. Its molecular mass is approximately 74 kDa. DNAI1 is expressed in a variety of tissues with higher expression observed in tissues involved in ciliary function. This protein is important for mediating cargo transport along microtubules contributing to cellular functions such as mitosis endocytosis and organelle positioning.
Biological function summary
Dynein intermediate chain 1 participates in key cellular processes linked to the movement of cilia and flagella. DNAI1 is an integral part of the axonemal dynein complex found in motile cilia. This complex enables the conversion of chemical energy from ATP into mechanical work driving the motion of these hair-like structures. Efficient cilia function relies on the coordinated action of axonemal dyneins enabling the clearance of mucus in respiratory pathways and movement of sperm in reproductive tracts.
Pathways
DNAI1 plays a role in the intraflagellar transport pathway important for building and maintaining cilia. Additionally DNAI1 is involved in the respiratory electron transport chain through its contribution to ciliary movement. The protein interacts closely with dynein heavy chain and dynein light chain proteins within these pathways ensuring the effective transport and movement of cellular components.
Defects in dynein intermediate chain 1 are linked to primary ciliary dyskinesia a genetic disorder affecting ciliary function. This disorder results in chronic respiratory tract infections and reduced fertility due to impaired ciliary motion. DNAI1 mutations can also connect to other ciliary dysfunctions impacting proteins like DNAH5 and DNAH11 which work alongside DNAI1 in the motility of cilia and flagella.