Abcam, ab249661, Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NADSYN1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10611,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249661 is the carrier-free version of
ab171942
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NADSYN1 also known as NAD synthetase 1 catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinic acid adenine dinucleotide (NaAD) and ammonium. The protein plays a critical role in NAD biosynthesis. NADSYN1 has a molecular mass of approximately 66 kDa and it is mostly expressed in the liver kidney and brain. These sites of expression highlight its importance in various tissue types where energy metabolism and cell signaling are important.
Biological function summary
NADSYN1 facilitates the production of NAD an important molecule in several metabolic processes. NAD is a coenzyme that is involved in redox reactions transferring electrons from one reaction to another. NADSYN1 functions as a monomer though it participates in larger biochemical pathways rather than forming a complex itself. Its activity ensures the availability of NAD which is necessary for efficient energy production and cellular functions.
Pathways
Its activity impacts the central NAD biosynthesis pathway. Nicotinamide adenine dinucleotide is essential in glycolysis and the citric acid cycle which are important for energy production in cells. NADSYN1 activity influences these pathways and it maintains cellular energy homeostasis. Alongside its involvement in NAD biosynthesis it has indirect relations with proteins like NAMPT in the salvage pathway of NAD synthesis as both serve the function of replenishing cellular NAD levels.
Abnormalities in NADSYN1 function can lead to metabolic disorders including congenital NAD deficiency disorders. Such disruptions can impact energy metabolism and lead to neurological and developmental issues. The dysfunction of NADSYN1 linked with oxidative stress disorders relates to its role in maintaining NAD levels which manage cellular redox states. The protein is essential in preventing energy deficits and minimizing the risk of disorders related to impaired NAD metabolism.