Abcam, ab249805, Anti-ETHE1 antibody [EPR11697] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ETHE1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11697,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249805 is the carrier-free version of
ab174302
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ETHE1 also known as persulfide dioxygenase plays an important role in the breakdown of sulfur-containing compounds. It catalyzes the conversion of persulfides to sulfite in the mitochondrial matrix. The protein has an approximate mass of 26 kDa and is strongly expressed in tissues with high metabolic activity such as the liver kidney and muscle. It plays an important role in maintaining cellular function and energy balance by efficiently processing by-products of cysteine metabolism.
Biological function summary
ETHE1 assists in detoxifying potentially harmful sulfur compounds. It forms a part of a complex with sulfide:quinone oxidoreductase (SQR) and thiosulfate sulfurtransferase (TST) to facilitate efficient sulfur metabolism. The importance of ETHE1 lies in its ability to prevent the accumulation of toxic sulfide levels which can impair cellular respiration and energy production. The protein's role as a persulfide dioxygenase places it in a unique position as one of the body's defenses against sulfide toxicity.
Pathways
ETHE1 has significant relevance in the sulfide oxidation pathway and cellular respiration processes. Particularly it interacts with SQR in the mitochondrial matrix to convert hydrogen sulfide to thiosulfate and sulfite which then enter the citric acid cycle. This critical pathway is associated with energy production and efficient cellular function. The cooperation between ETHE1 and SQR ensures that toxic sulfide levels do not rise protecting mitochondrial integrity.
Defects in ETHE1 result in ethylmalonic encephalopathy a severe metabolic disorder. The lack of functional ETHE1 leads to the accumulation of toxic levels of hydrogen sulfide which may disrupt mitochondrial function and energy production. This disorder highlights the interconnectedness of ETHE1 with SQR as impairments in this protein complex can exacerbate the pathological condition. Understanding the role of ETHE1 in this disease helps target therapies aimed at reducing sulfide toxicity.