Abcam, ab2501, Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6]

Size: 50µg
Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501) is a rat monoclonal antibody detecting Heparan Sulfate Proteoglycan 2/Perlecan in Western Blot, IP, IHC-P, IHC-Fr . Suitable for Cow, Fish, Human, Mouse, Pig, Rat . - Over 30 publications - Trusted since 2002
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:A7L6,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human, Rat, Mouse, Cow, Fish, Pig,
Applications:IHC-Fr, IHC-P, WB, ICC, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Tissue preparation containing Basement membrane-specific heparan sulfate proteoglycan core protein protein. The exact immunogen used to generate this antibody is proprietary information.P98160,
Specificity:Recognizes domain IV of heparan sulphate proteoglycan 2 / perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.

Product details:
What is this antibody validated in?
Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Cow, Fish, Human, Mouse, Pig, Rat samples.
Trusted by the scientific community
Anti-Heparan Sulfate Proteoglycan 2/Perlecan [A7L6] (ab2501) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-Heparan Sulfate Proteoglycan 2/Perlecan [A7L6] (ab2501) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.
Biological function summary
Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.
Pathways
Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.
Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.