Abcam, ab250169, Anti-MRPL4 antibody [EPR13151] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MRPL4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13151,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250169 is the carrier-free version of
ab180165
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRPL4 also known as 39S ribosomal protein L4 mitochondrial is a mitochondrial ribosomal protein. This protein is a component of the large 39S subunit of the mitochondrial ribosome and has a molecular mass of approximately 39 kDa. MRPL4 is primarily expressed in tissues with high energy demands including the heart and skeletal muscle. It plays a role in mitochondrial protein synthesis facilitating the translation of proteins encoded by mitochondrial DNA.
Biological function summary
MRPL4 assists in the assembly and stability of the mitochondrial ribosome forming part of the mitoribosome complex. This complex is essential for the synthesis of mitochondrial proteins which are critical for proper mitochondrial function. MRPL4 ensures accurate translation by binding to ribosomal RNA stabilizing the structure needed for efficient ribosomal activity. Its function is important for maintaining cellular energy production cellular growth and metabolism.
Pathways
MRPL4 participates significantly in the mitochondrial translation pathway. This pathway is integral to the oxidative phosphorylation process which is essential for ATP production in cells. MRPL4 interacts with other ribosomal proteins such as MRPL3 and MRPL12 facilitating the coordination of protein synthesis within the mitochondrion. This process influences cellular respiration and bioenergetics which are critical for adapting to energy requirements.
MRPL4 is associated with conditions like mitochondrial disorders and cardiac diseases. Alterations in MRPL4 expression or function can disrupt mitochondrial protein synthesis leading to impaired oxidative phosphorylation and energy production. Such mitochondrial dysfunction is linked to disorders like mitochondrial encephalomyopathy and cardiomyopathy. MRPL4's interactions with other ribosomal proteins ensure that changes in its structure or expression could influence disease progression making it a target for further research in mitochondrial medicine.