Abcam, ab250198, Anti-PSMC5 antibody [EPR13566(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PSMC5 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13566(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250198 is the carrier-free version of
ab180495
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PSMC5 also known as Tbp1 or Rpt6 is a component of the 26S proteasome complex that functions in the degradation of ubiquitinated proteins. This protein has a molecular mass of approximately 44 kDa. It shows a widespread pattern of expression being present in various tissues across the human body particularly in tissues with high metabolic activity. As an ATPase associated with diverse cellular activities the main mechanical function of PSMC5 is driving protein unfolding which is essential for proteasome activity.
Biological function summary
PSMC5 is integral to maintaining protein homeostasis and regulating protein turnover within cells. It forms part of the regulatory particle of the proteasome complex engaging in the recognition and unfolding of polyubiquitinated substrates. This action facilitates the degradation process important for numerous cellular processes including cell cycle apoptosis and DNA damage response. The presence of PSMC5 in these pathways indicates its involvement in pivotal cellular functions that regulate cell health and survival.
Pathways
PSMC5 plays a fundamental role in the ubiquitin-proteasome pathway a critical system for protein degradation and turnover. This pathway regulates the concentration of several key regulatory proteins therefore maintaining cellular homeostasis. PSMC5 interacts with other core proteasome components including PSMC1 and PSMC2. Additionally it contributes to the DNA repair pathways by interacting with proteins involved in genotoxic stress response underlining its significance in maintaining genomic stability.
PSMC5 activity has associations with disorders such as cancer and neurodegenerative diseases. Dysregulation of the proteasome function may lead to aberrant protein accumulation which constitutes a factor in the development of these diseases. In cancer PSMC5 can interact with oncogenic pathways such as those involving proteins like MDM2 a ubiquitin ligase influencing tumorigenesis. Similarly in neurodegenerative disorders PSMC5's role in proteostasis connects it to proteins such as ubiquitin impacting disease progression.