Abcam, ab250297, Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RNF20 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13563(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250297 is the carrier-free version of
ab181032
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF20 also known as BRE1A or E3 ubiquitin-protein ligase RNF20 mechanically functions as an E3 ligase that mediates ubiquitination. Its mass is approximately 110 kDa. RNF20 is expressed across various tissues with a high presence in the spleen and testis. It specifically adds ubiquitin to lysine residues on target histones leading to changes in chromatin structure and gene expression.
Biological function summary
RNF20 works within the ubiquitination system and forms part of the RNF20/RNF40 complex. This complex plays a role in the monoubiquitination of histone H2B at lysine 120 (H2BK120ub) a significant post-translational modification influencing transcriptional regulation. The modification aids in recruiting downstream effectors and is important for gene expression regulation and DNA damage repair mechanisms.
Pathways
RNF20 interacts with several cellular processes including the DNA damage response and transcriptional activation pathways. It significantly contributes to the DNA repair pathway by facilitating recruitment of repair proteins and is also involved in transcription regulation through the histone modification pathway. Furthermore RNF20 shows a functional relationship with RNF40 another E3 ligase in these pathways emphasizing its integrative role in cellular functioning.
Disruptions in RNF20 activity have connections to cancer and neurodegenerative disorders. Altered RNF20 expression or mutations can influence tumor growth due to impaired regulation of gene expression and DNA repair impacting proteins like p53. Additionally in neurodegenerative disorders anomalies in RNF20-mediated ubiquitination can affect protein homeostasis linking to proteins involved in neuronal integrity and survival.