Abcam, ab250535, Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal alpha A Crystallin/CRYAA antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14125(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250535 is the carrier-free version of
ab181866
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha A Crystallin also known as CRYAA or crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is the major structural protein in the eye lens and is also expressed in other tissues like the heart kidney and brain. The protein forms large complexes with other alpha crystallin proteins to provide structural stability and help maintain the transparency and refractive index of the lens. This specific role of alpha A Crystallin makes it essential for proper eye function.
Biological function summary
Alpha A Crystallin acts like a molecular chaperone. It helps prevent the aggregation of misfolded proteins which is important in maintaining cellular protein homeostasis. Alpha A Crystallin can combine with alpha B Crystallin in lens fiber cells to form a hetero-oligomeric complex. This ability to bind unfolded proteins and assist in refolding processes highlights its importance in cell stress responses especially in situations where proteins could potentially denature and lose function.
Pathways
Alpha A Crystallin participates significantly in the oxidative stress response and apoptotic pathways. It interacts with proteins involved in these processes such as caspases to inhibit cell death and curb the deleterious effects of reactive oxygen species. In the apoptotic pathway alpha A Crystallin's role in regulating cell survival highlights its association with proteins like Hsp70 another heat shock protein that serves as a stabilizing agent during cellular stress.
Certain mutations in alpha A Crystallin link it to cataracts and age-related macular degeneration. These ocular conditions arise when the protein's chaperone function declines leading to the aggregation of lens proteins and subsequent loss of lens transparency. Alpha B Crystallin the sister protein shows a similar role in these disorders indicating how disturbances in the intricate network of crystallin interactions affect ocular health.