Abcam, ab250564, Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RNF185 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14070-94,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250564 is the carrier-free version of
ab181999
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF185 also known as RING finger protein 185 is an E3 ubiquitin-protein ligase that plays an important role in the ubiquitination process. It works with RNF5/NG2 another protein and both belong to the RING finger protein family which is characterized by a specific domain that facilitates protein-protein interactions. This protein has a mass of approximately 35 kDa. RNF185 is expressed across various tissues with higher levels often noted in the heart and skeletal muscle indicating a role in cell function within these tissues.
Biological function summary
RNF185 regulates protein homeostasis by marking target proteins for degradation via ubiquitination. It does not act alone; often it forms part of a larger complex that includes components like E2 ubiquitin-conjugating enzymes. This activity ensures that cells maintain the proper levels of proteins helping remove misfolded or damaged proteins. The complex activity of RNF185 is essential for cellular quality control mechanisms maintaining balance within the protein environment of the cell.
Pathways
Involvement of RNF185 connects it to important cellular processes including the endoplasmic reticulum-associated degradation (ERAD) pathway and the Wnt signaling pathway. In ERAD RNF185 collaborates with other proteins such as RNF5 to facilitate degradation of misfolded proteins in the endoplasmic reticulum. Through the Wnt pathway control of protein levels by RNF185 influences cellular proliferation and differentiation processes that are essential for proper tissue development and homeostasis.
RNF185 shows significant links to cardiovascular diseases and some forms of cancer. Dysregulation of RNF185 and its interaction with proteins like RNF5 can lead to abnormal protein accumulation or degradation contributing to pathogenesis in these conditions. In cardiovascular diseases improper protein turnover affecting heart and muscle tissues can lead to cardiac dysfunction. In cancer altered activity of RNF185 may affect cell growth pathways promoting tumor growth and progression.