Abcam, ab250835, Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ABCE1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15373(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human, Rat,
Applications:Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

Product details:
ab250835 is the carrier-free version of
ab185548
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ABCE1 also known as ATP-binding cassette sub-family E member 1 is an ATPase involved in various cellular processes. It belongs to the ATP-binding cassette (ABC) transporter family. The protein mass is approximately 68 kDa and its expression occurs widely in various tissues including the cytoplasm and mitochondria. ABCE1 has a unique structure with two nucleotide-binding domains making it distinct from other ABC family members that typically transport molecules across membranes.
Biological function summary
ABCE1 plays a role in inhibiting ribonuclease activity and is essential in ribosome recycling. This protein interacts with translation initiation factors and actively participates in the dissociation of ribosomes after translation termination. It is a part of the ribosome recycling complex an important multi-proteins setup necessary to prepare ribosomes for another round of translation. The biochemical interactions and presence in different cellular compartments highlight its multiple roles in cellular homeostasis.
Pathways
ABCE1 engages significantly in translation and its regulation. It plays an important role in the translation termination pathway where it interacts with eukaryotic release factors. Additionally it influences the degradation pathway of defective ribosomal products. Within these pathways the function of ABCE1 is closely linked to proteins like eRF1 and eRF3 which are essential in the translation termination process. The interaction of these proteins ensures efficient termination and recycling of translation machinery components.
Alterations in ABCE1 expression or function link to certain cancers and viral infections. The aberrant activity of ABCE1 influences cancer progression as it affects protein synthesis and cellular growth levels. Also ABCE1 has been implicated in promoting the replication of certain viruses providing them a competitive advantage. Proteins like eIF3 and eIF4 involved in translation initiation can show altered associations in disorders where ABCE1 is dysregulated influencing disease outcomes and progression.