Abcam, ab250869, Anti-FGD2 antibody [EPR15306] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FGD2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15306,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250869 is the carrier-free version of
ab185968
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FGD2 also known as FYVE RhoGEF and PH domain-containing protein 6 (FGD1) is a protein with a mass of about 92 kDa. FGD2 functions mechanically as a guanine nucleotide exchange factor (GEF) that activates Rho GTPases. This protein is mostly expressed in hematopoietic tissues including the spleen and lymph nodes. FGD2 facilitates actin cytoskeleton organization which is essential for various cellular processes.
Biological function summary
FGD2 has a role in cellular signaling and cytoskeletal dynamics. It regulates the activation of Rho GTPases such as Cdc42 a small signaling G protein. FGD2 does not function within a known protein complex but partners with other signaling molecules to influence cell shape and movement. This biological activity affects how cells respond to external stimuli.
Pathways
FGD2 is involved in regulating cytoskeleton organization and cell signaling pathways. In the context of the Wnt signaling pathway FGD2 interacts with proteins that modulate cytoskeletal rearrangement such as Rac1 and Cdc42. These interactions suggest FGD2 influences pathways critical for cell division and differentiation.
Abnormalities in FGD2 are related to immune system disorders and some cancers. Dysregulated FGD2 expression associates especially with Hodgkin's lymphoma where it affects actin polymerization and cell migration. Additionally alterations in FGD2 interact with beta-catenin in Wnt signaling pathways related to tumorigenesis. Understanding FGD2's role can provide insights into therapeutic strategies for disorders linked to actin cytoskeletal defects.