Abcam, ab250883, Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Elav-type RNA-binding protein ETR3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13374-2,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-P, IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250883 is the carrier-free version of
ab186430
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Elav-type RNA-binding protein ETR3 also known as CELF1 (CUGBP Elav-like family member 1) is involved in the regulation of pre-mRNA alternative splicing translation and stability. The protein has a mass of approximately 52 kDa. ETR3 is expressed in several tissues with higher levels found in the heart skeletal muscles and brain. These tissues are indicative of its important role in post-transcriptional mechanism. As a member of the ELAV (embryonic lethal abnormal vision)-like proteins ETR3 contains three RNA recognition motifs which mediate its binding to RNA.
Biological function summary
ETR3 influences various cellular processes by participating in RNA processing and nuclear-cytoplasmic transport. It forms part of ribonucleoprotein complexes that interact with target RNA impacting splicing decisions and gene expression levels. ETR3's activity is important for muscle and neuronal function helping modulate the expression of genes vital for muscle contraction and neuronal communication. Through its role as an RNA-binding regulator it can affect cell differentiation and growth.
Pathways
ETR3 plays significant roles in the regulation of alternative splicing and mRNA decay pathways. It interacts with several other splicing and mRNA degradation factors such as hnRNPH (heterogeneous nuclear ribonucleoprotein H) and PABPN1 (poly(A) binding protein nuclear 1). These interactions help control the turnover and translation of mRNA establishing a balance of protein synthesis important for maintaining cellular homeostasis. By influencing these pathways ETR3 impacts cardiac and neural-specific gene expression demonstrating its role in tissue-specific regulation.
ETR3 is linked to myotonic dystrophy and cardiac arrhythmias. ETR3 misregulation leads to disruptions in alternative splicing of specific mRNAs contributing to the pathophysiology of these conditions. The relationship between ETR3 and proteins such as MBNL1 (Muscleblind-like splicing regulator 1) in myotonic dystrophy is particularly significant. Both proteins regulate alternative splicing and their misregulated expression in disease highlights an important area for potential therapeutic interventions. The association of ETR3 with such disorders highlights its relevance in maintaining normal cellular and physiological functions.