Abcam, ab250907, Anti-GTF2E2 antibody [EPR14929] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GTF2E2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14929,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250907 is the carrier-free version of
ab187143
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target GTF2E2 also known as General Transcription Factor IIE Subunit 2 participates in the initiation of transcription by RNA polymerase II. It plays an important role in the formation of the transcription initiation complex. The protein has a molecular mass of approximately 32 kDa. GTF2E2 is expressed in various tissues with significant expression detected in the human heart and skeletal muscle.
Biological function summary
GTF2E2 interacts with other general transcription factors to promote the assembly of the pre-initiation complex. It forms a part of the TFIIE complex with another subunit TFIIE α. This complex is significant for transcription initiation as it helps in opening the DNA double helix. Its activity influences gene expression regulation and is important for directing RNA polymerase II to specific promoter sites.
Pathways
The protein is involved in the RNA polymerase II transcription initiation pathway a critical step for mRNA synthesis. Additionally it affects the regulation of the p53 signaling pathway often interacting with proteins like TFIIH and TBP. The association with these pathways highlights its role in maintaining the gene expression safety and responding to cellular stress signals.
Altered expression of GTF2E2 correlates with cancer and developmental disorders. Its dysfunction might lead to malformed transcription initiation influencing oncogenic pathways. Mutations or misregulation can affect the stability of the transcription machinery potentially linking it to the BRCA1 protein in breast cancer further highlighting the need for precise transcription control.