Abcam, ab250916, Anti-USP25 antibody [EPR15019] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal USP25 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15019,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250916 is the carrier-free version of
ab187156
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP25 also known as Ubiquitin Specific Peptidase 25 is a deubiquitinating enzyme that removes ubiquitin moieties from polyubiquitinated proteins. It has a molecular mass of approximately 120 kDa. This protein is expressed in various tissues with notable expression in the brain kidney and thymus. USP25 plays an essential role in regulating the balance between protein ubiquitination and deubiquitination which is critical for maintaining cellular protein homeostasis.
Biological function summary
USP25 contributes to the modulation of several key proteins by rescuing them from proteasomal degradation through deubiquitination. This protein is involved in multiple cellular processes such as signal transduction and the immune response. USP25 is part of the endoplasmic reticulum-associated degradation (ERAD) complex where it functions to recycle ubiquitin molecules from substrates destined for degradation. By doing so USP25 influences the stability and localization of various proteins impacting cell growth and survival.
Pathways
USP25 is involved in the ubiquitin-proteasome system (UPS) and the Wnt signaling pathway. In the UPS USP25 interacts with proteins like LRP6 helping regulate the amount of ß-catenin thereby influencing Wnt signaling activity. This interaction plays a role in controlling cell proliferation and differentiation. USP25 also associates with other deubiquitinating enzymes reflecting its role in multiple regulatory pathways essential for cellular function.
Research links USP25 to inflammatory diseases and certain cancers. Its role in cancer is as a regulator of tumor-suppressor proteins and oncogenes such as those involving p53 pathways. Inflammatory conditions like rheumatoid arthritis show altered USP25 expression patterns where it interacts with proteins like TNF-alpha receptor-associated factors (TRAFs) impacting inflammatory signaling cascades. Studies continue to explore USP25's potential as a therapeutic target in these disorders.