Abcam, ab250963, Anti-GATAD2A antibody [EPR18252] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GATAD2A antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18252,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250963 is the carrier-free version of
ab188472
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GATAD2A gene encodes a protein also known as P66-alpha weighing approximately 65 kDa. This protein is largely expressed in the nucleus of eukaryotic cells. GATAD2A interacts with other proteins to regulate transcription and is part of the transcription repression complexes. Its expression is seen widely across various tissues indicating its broad regulatory role in gene expression.
Biological function summary
GATAD2A functions by interacting with multiple factors to form the nucleosome remodeling and deacetylase (NuRD) complex. This complex is critical in modifying chromatin to control gene transcription. GATAD2A assists in recruiting this complex to specific genomic regions helping to repress or activate gene expression depending on the cellular context. GATAD2A's involvement in chromatin remodeling highlights its important role in cell cycle regulation and development.
Pathways
GATAD2A participates in epigenetic regulation pathways and the cellular differentiation process. It cooperates with other NuRD complex proteins like MBD2 and CHD4 which play roles in DNA methylation and histone modification. Through these interactions GATAD2A helps in maintaining the balance between gene repression and activation which is necessary for various cellular processes including differentiation and proliferation.
GATAD2A shows associations with neurodevelopmental disorders and certain cancer forms. It has been noted to interact with proteins involved in these conditions such as transcriptional repressors and factors like CDK11 suggesting its role in disease progression. Abnormal GATAD2A function or expression can lead to disrupted chromatin dynamics potentially contributing to the pathogenesis of these disorders.