Abcam, ab251104, Anti-NDUFS2 antibody [EPR16266] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NDUFS2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16266,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251104 is the carrier-free version of
ab192022
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFS2 also known as NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 is a significant component of complex I in the mitochondrial respiratory chain. This protein has a mass of approximately 54 kDa and is primarily found in mitochondrial membranes of cells across various tissues. It plays an essential role in electron transfer working closely with other subunits to facilitate the conversion of NADH to NAD+ contributing to the process of oxidative phosphorylation.
Biological function summary
NDUFS2 serves an important role in energy production as part of the multi-subunit complex I which is also known as NADH:ubiquinone oxidoreductase. This complex catalyzes the first step in the mitochondrial electron transport chain transferring electrons from NADH to ubiquinone. Through this process NDUFS2 helps power ATP synthesis by contributing to the establishment of a proton gradient across the inner mitochondrial membrane which is fundamental to cellular energy metabolism.
Pathways
The function of NDUFS2 is integral to both the oxidative phosphorylation and citric acid cycle pathways. Within oxidative phosphorylation NDUFS2 associates closely with other complex I subunits such as NDUFS1 and NDUFV1. These associations allow it to effectively participate in the electron transport chain facilitating energy production. The citric acid cycle indirectly impacts NDUFS2's function by supplying NADH which is necessary for complex I activity and efficient energy conversion.
NDUFS2 mutations and dysfunctions relate to disorders such as Leigh syndrome and some mitochondrial myopathies. Leigh syndrome a severe neurological disorder often involves deficiencies in complex I where NDUFS2 plays a significant role. This protein also shows connections to other mitochondrial proteins like NDUFA12 and NDUFB6 which when also impaired can contribute to disease manifestations. The study and understanding of NDUFS2's role in these conditions are important for developing potential therapeutic strategies.