Abcam, ab251110, Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free

Size: 100µg / 1mg
Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Nectin-4 in Western Blot, IHC-P . Suitable for Human . - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15613-68,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Customers should note, the current antibody has been examined with alternative automated IHC staining platforms and optimal results were obtained using the Ventana DISCOVERY ULTRA with associated kits and reagents. We were not able to obtain optimal results with Leica BOND platform when using the Polymer Refine detection kit, 20min ER1 antigen retrieval and 16min antibody incubation at room temperature. Further optimisation may be needed when using the Leica BOND platform.The antibody detected a 50kDa and 70kDa non-specific band in the negative sample MDA-MB-231, which are close to the target band. Therefore, we recommend including a negative sample when performing Western Blot analysis.We suggest using the whole cell and cell supernatant lysate side by side to observe the expression of different form of Nectin-4. The cell supernatant can detect soluble Nectin-4 mainly (PMID: 15784625).Negative control: MDA-MB-231 (15784625, PMID: 31617074, PMID: 24386110, PMID: 17474988).

Product details:
What is this antibody validated in?
Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of Nectin-4?
Anti-Nectin-4 [EPR15613-68] - BSA and Azide free (ab251110) specifically detects a band for Nectin-4 (UniProt: Q96NY8) at a molecular weight of 55kDa.
Other related products
We have a range of other formats of antibody clone [EPR15613-68] also available for your convenience:
ab192033
, Carrier free - ab251110
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nectin-4 also known as PVRL4 is a member of the immunoglobulin-like adhesion molecule family. It has a molecular weight of approximately 66 kDa. Nectin-4 plays an important role in cell-cell adhesion by forming trans-dimers promoting cellular cohesion. It is expressed in tissues such as placenta embryonic tissues and several epithelial cancers indicating its potential involvement in developmental processes and pathological conditions. When utilizing methods such as 'nectin-4 ELISA' and 'nectin-4 IHC' researchers can study its expression extensively across different tissue samples.
Biological function summary
Nectin-4 functions as an important adhesion molecule that contributes to the maintenance and organization of cell junctions. It is often part of larger adhesive complexes that include afadin which links nectin proteins to the actin cytoskeleton stabilizing cell structure. Besides its role in maintaining cell-cell adhesion nectin-4 is significant in processes like cellular polarization and migration. These properties make it an important target for understanding tissue development and integrity as well as cancer metastasis.
Pathways
Nectin-4 belongs to the pathways regulating cell adhesion and the cytoskeletal organization. It is closely associated with the cadherin and integrin signaling pathways where it collaborates with other nectin family members and afadin to regulate these networks. These interactions impact cellular processes such as signal transduction and intercellular communication critical for tissue maintenance. Studies involving 'nectin expression' help further elucidate these pathway connections and their implications in healthy and diseased tissues.
Nectin-4 shows significant correlation with certain cancers such as breast and lung carcinoma. Its elevated expression in tumor tissues suggests a role in tumorigenesis and cancer progression. The protein's interaction with oncogenes like HER2 indicates a possible pathway by which nectin-4 affects cancer cell proliferation and survival. Understanding 'nectin-4 expression' in the context of these diseases aids in identifying potential biomarkers and therapeutic targets for cancer diagnosis and treatment.