Abcam, ab251125, Anti-Pigu antibody [EPR16424] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PIGU antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16424,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251125 is the carrier-free version of
ab192255
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target known as PIG-U also referred to as Phosphatidylinositol Glycan Anchor Biosynthesis Class U is an essential component in cellular processes. PIG-U is part of the glycosylphosphatidylinositol (GPI) transamidase complex important for GPI-anchor biosynthesis. This process allows proteins to attach to membranes essential for cell surface functionality. The approximate molecular mass of PIG-U is 50 kDa. It is widely expressed in various tissues especially those with high rates of cell division and growth.
Biological function summary
PIG-U plays an important role in enabling proteins to attach to the cell membrane via GPI anchors. This protein forms a part of a multi-subunit complex including other critical proteins like PIG-S and PIG-T. The function of PIG-U ensures that proteins needing a GPI-anchor for their localization and function on cellular membranes get tethered correctly. GPI-anchored proteins are involved in cell signaling cell adhesion and interactions with the extracellular matrix highlighting the functional diversity PIG-U supports.
Pathways
GPI-anchor synthesis involves PIG-U as a central node in the GPI biosynthesis pathway. This pathway is essential for the post-translational modification of proteins that will become GPI-anchored. An important protein associated with PIG-U through this pathway is the GPI-Anchor Transamidase complex. Another significant pathway related to PIG-U is the N-Glycan biosynthesis where proper glycosylation of proteins is coordinated sharing a network with proteins like GnT-1.
Mutations or dysfunctions in PIG-U have links to the pathogenesis of Paroxysmal Nocturnal Hemoglobinuria (PNH) which involves the body's inability to regulate complement on the surface of blood cells. Additionally various forms of intellectual disability relate to abnormalities in the GPI-anchor biosynthesis pathway influenced by PIG-U's functionality. PIG-T and other components of the GPI Transamidase complex are also implicated in these disorders highlighting the importance of PIG-U in maintaining normal cellular function and health.