Abcam, ab251238, Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TrkA phospho Y496 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19140,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human,
Applications:Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251238 is the carrier-free version of
ab197071
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TrkA TrkB and TrkC also known as Ntrk1 Ntrk2 and Ntrk3 respectively are receptor tyrosine kinases expressed mainly in neurons of the central and peripheral nervous systems. TrkA has a mass of about 140 kDa TrkB about 145 kDa and TrkC around 140 kDa. These receptors bind to neurotrophins: TrkA interacts with nerve growth factor (NGF) TrkB with brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and TrkC with neurotrophin-3 (NT-3). Their primary role is to mediate neurotrophin signaling resulting in activation of downstream signaling cascades that support neuronal survival development and function.
Biological function summary
TrkA TrkB and TrkC play critical roles in nervous system development and maintenance. These receptors upon binding with their respective ligands form dimers which leads to autophosphorylation on specific tyrosine residues. This activation recruits adaptor proteins and through a series of interactions initiates multiple signaling pathways. Among these are the MAPK PI3K and PLCγ pathways which further propagate signals that regulate cell differentiation growth and survival demonstrating their importance in neurobiology.
Pathways
These Trk receptors engage in important signaling pathways that include the MAPK/ERK pathway and the PI3K/Akt pathway. These pathways are major components of cellular functions like proliferation differentiation and survival. During activation Trk receptors can interact with proteins like SHC and GRB2 within these pathways. Their signaling cascades influence neuronal plasticity and repair indicating their vital involvement in cellular responses to environmental stimuli.
TrkA TrkB and TrkC serve important roles in conditions like neurodegenerative diseases and certain cancers. For instance altered Trk receptor signaling has been associated with Alzheimer's disease where BDNF signaling via TrkB is often impaired. Similarly mutations or dysfunctional signaling of these receptors are implicated in cancers such as neuroblastoma where TrkA can act as an oncogene. These disorders highlight the relationship of these receptors to other proteins like tau in Alzheimer's disease and p75NTR in neuronal growth and cancerous transformations.